primary antibodies phf-1 Search Results


primary antibodies of at phf1  (Agrisera)
90
Agrisera primary antibodies of at phf1
Immunoblot analysis of root microsomal proteins extracted from WT, cnih5 , and pho2 mutants. (A and B) Protein expression of At <t>PHF1,</t> At PHO1, and At NRT2.1 (A) and At CNIH5 (B) in the root of 11-day-old seedlings of Arabidopsis WT, cnih5 , and pho2 under Pi-sufficient (+P) and Pi-deficient (–P, 0 µM KH 2 PO 4 , five days of starvation) conditions. The band of At CNIH5 is indicated by arrowhead. The relative protein expression level was normalized with the corresponding amido black staining and relative to the WT control. Error bars represent SE (n = 2–4). The applied extraction methods were shown as indicated.
Primary Antibodies Of At Phf1, supplied by Agrisera, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Immunoblot analysis of root microsomal proteins extracted from WT, cnih5 , and pho2 mutants. (A and B) Protein expression of At PHF1, At PHO1, and At NRT2.1 (A) and At CNIH5 (B) in the root of 11-day-old seedlings of Arabidopsis WT, cnih5 , and pho2 under Pi-sufficient (+P) and Pi-deficient (–P, 0 µM KH 2 PO 4 , five days of starvation) conditions. The band of At CNIH5 is indicated by arrowhead. The relative protein expression level was normalized with the corresponding amido black staining and relative to the WT control. Error bars represent SE (n = 2–4). The applied extraction methods were shown as indicated.

Journal: bioRxiv

Article Title: CORNICHON HOMOLOG 5-dependent ER export of membrane cargoes in phosphate-starved Arabidopsis root as revealed by membrane proteomic analysis

doi: 10.1101/2024.12.30.630708

Figure Lengend Snippet: Immunoblot analysis of root microsomal proteins extracted from WT, cnih5 , and pho2 mutants. (A and B) Protein expression of At PHF1, At PHO1, and At NRT2.1 (A) and At CNIH5 (B) in the root of 11-day-old seedlings of Arabidopsis WT, cnih5 , and pho2 under Pi-sufficient (+P) and Pi-deficient (–P, 0 µM KH 2 PO 4 , five days of starvation) conditions. The band of At CNIH5 is indicated by arrowhead. The relative protein expression level was normalized with the corresponding amido black staining and relative to the WT control. Error bars represent SE (n = 2–4). The applied extraction methods were shown as indicated.

Article Snippet: Membrane was blocked with 1–2% BSA in 1× PBS solution with 0.2% Tween 20 (PBST, pH 7.2) at RT for 1 h, and rinsed two times with distilled water followed by hybridization with primary antibodies of At PHF1 , At PHO1 , At NRT2.1 (AS12 2612; Agrisera), and At CNIH5 at RT for 2 h or at 4 °C overnight in blocking solution.

Techniques: Western Blot, Expressing, Staining, Control, Extraction